Edgar Specker, FMP, Berlin-Buch
The compound management as part of the MedChem group at the Leibniz Institut für Molekulare Pharmakologie (FMP) assures optimal control quality and identity of each compound in the screening library. Thereto a routine and robust procedure was developed which ensures a qualified assessment of compounds in terms of their purity and stability. Compounds are transferred via SOP´s to the automated storage system (KIWI Store from Liconic AG). If compounds were identified as hits in the primary screen a new batch of compounds could be ordered and used for the validation in the hit picking process. A small aliquot is separated from the hit picking plates and measured via LC-MS. The qualitative and quantitative analysis by means of UV and MS spectroscopy is an important feature for the evaluation and confirmation of a selected hit.
EU-OPENSCREEN (www.eu-openscreen.eu) supports the development of novel chemical research tools for the Life Sciences community by providing transnational open access to the most advanced technologies, chemical and biological resources, and expertise, including:
- Access to a unique European compound collection containing commercial as well as proprietary compounds collected from chemists from all over Europe
- High-throughput screening of this collection for the discovery of bioactive substances (so-called ‘hit’ compounds) carried out by a network of state-of-the-art high-throughput screening facilities
- Chemical optimization of these preliminary ‘hits’ to translate them into valuable (i.e. selective, potent) research tools
- Broad compound profiling services to comprehensively characterise (physicochemical and biological properties) these research tools before they are widely utilised by the scientific community.
