Holger Erfle, Ruprecht-Karls-Universität Heidelberg
Delivery of large and functionally active biomolecules across cell membranes is a challenge, particularly for high-throughput experiments. For this purpose, we developed a novel solid-phase reverse transfection method that is suitable for the intracellular delivery of large molecules, such as proteins and antibodies, into mammalian cells with preservation of their function.
I will present for three highly diverse application areas of the method, ranging from antibody-mediated protein localization and antibody-mediated inhibition of protein function to CRISPR/Cas9 based gene editing in living cells. The method is scalable to high-throughput and enables pre-fabrication of “ready to transfect” multiwell plates or cell microarrays carrying thousands of proteins or antibodies. This allows their easy distribution and standardization of biological assays across different laboratories.
Bulkescher R, Starkuviene V, Erfle H. Solid-phase reverse transfection for intracellular delivery of functionally active proteins. Genome Res. 2017 Oct;27(10):1752-1758. doi: 10.1101/gr.215103.116. Epub 2017 Sep 5.
Starkuviene V, Kallenberger SM, Beil N, Lisauskas T, Schumacher BS, Bulkescher R, Wajda P, Gunkel M, Beneke J, Erfle H.High-Density Cell Arrays for Genome-Scale Phenotypic Screening. SLAS Discov 2019 Jan 25:2472555218818757. doi: 10.1177/2472555218818757.
