Stefan Müller, Evotec

Evotec’s chemoproteomic approaches to deconvolute target proteins of small molecules are unbiased, address the entire cellular proteome, and are applicable in various organisms. Target proteins are analysed by quantitative mass spectrometry, which can be based on different quantitation strategies.

Assisted by SAR information the chemical entities are modified with short linker structures that allow for multiple applications from target identification, binding site identification, to target engagement assays in follow-up studies. For target deconvolution such derivatives are applied for affinity chromatography, or for covalent target capture by photoaffinity labeling, or activity-based protein profiling.

General workflows and different case studies will be presented during the talk.